All of a sudden non of my users can connect - error: wrong password

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mcpeterc
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All of a sudden non of my users can connect - error: wrong password

Hi.

I have a Windows server 2016 that runs a IIS ftp site. My users connect via port 990 and all worked fine a couple of days ago. Now when they log on I get this error:
"TLS connection stablished. Waiting fir welcome message... Access denied"

I checked the following:
- all users have write/write access to their own home-folder. Both on OS level and within IIS
- reset the password so im 100% positive that the PW is the right one.
- Turned off the local firewall


I use User isolation. I tried to give 'All Users' Read,Write access - that did not help
Can anyone help?

This is the log
log:
* 2020-03-05 12.35.03.724 (ESshFatal) 
. 2020-03-05 12.35.05.965 --------------------------------------------------------------------------
. 2020-03-05 12.35.05.966 WinSCP Version 5.15.3 (Build 9730) (OS 10.0.17763 - Windows 10 Enterprise)
. 2020-03-05 12.35.05.966 Configuration: HKCU\Software\Martin Prikryl\WinSCP 2\
. 2020-03-05 12.35.05.966 Log level: Normal
. 2020-03-05 12.35.05.966 Local account: DESKTOP-VEK9I3I\Admin
. 2020-03-05 12.35.05.966 Working directory: C:\Program Files (x86)\WinSCP
. 2020-03-05 12.35.05.966 Process ID: 15740
. 2020-03-05 12.35.05.966 Command-line: "C:\Program Files (x86)\WinSCP\WinSCP.exe" 
. 2020-03-05 12.35.05.966 Time zone: Current: GMT+1, Standard: GMT+1 (Romance Standard Time), DST: GMT+2 (Romance Daylight Time), DST Start: 29/03/2020, DST End: 25/10/2020
. 2020-03-05 12.35.05.967 Login time: Thursday, 5 March 2020 12.35.05
. 2020-03-05 12.35.05.967 --------------------------------------------------------------------------
. 2020-03-05 12.35.05.967 Session name: xxxx@89.xxxxxxx (Site)
. 2020-03-05 12.35.05.967 Host name: 89.xxxxxxxx (Port: 990)
. 2020-03-05 12.35.05.967 User name: xxxxx (Password: Yes, Key file: No, Passphrase: No)
. 2020-03-05 12.35.05.967 Transfer Protocol: FTP
. 2020-03-05 12.35.05.967 Ping type: Dummy, Ping interval: 30 sec; Timeout: 15 sec
. 2020-03-05 12.35.05.967 Disable Nagle: No
. 2020-03-05 12.35.05.967 Proxy: None
. 2020-03-05 12.35.05.967 Send buffer: 262144
. 2020-03-05 12.35.05.967 UTF: Auto
. 2020-03-05 12.35.05.967 FTPS: Implicit TLS/SSL [Client certificate: No]
. 2020-03-05 12.35.05.967 FTP: Passive: Yes [Force IP: Auto]; MLSD: Auto [List all: Auto]; HOST: Auto
. 2020-03-05 12.35.05.967 Session reuse: Yes
. 2020-03-05 12.35.05.967 TLS/SSL versions: TLSv1.0-TLSv1.2
. 2020-03-05 12.35.05.967 Local directory: C:\Users\Admin\Documents, Remote directory: /, Update: Yes, Cache: Yes
. 2020-03-05 12.35.05.967 Cache directory changes: Yes, Permanent: Yes
. 2020-03-05 12.35.05.967 Recycle bin: Delete to: No, Overwritten to: No, Bin path: 
. 2020-03-05 12.35.05.967 Timezone offset: 0h 0m
. 2020-03-05 12.35.05.967 --------------------------------------------------------------------------
. 2020-03-05 12.35.06.005 Connecting to 89.xxxxxxx:990 ...
. 2020-03-05 12.35.06.005 Connected with 89.xxxxxxxxx:990, negotiating TLS connection...
. 2020-03-05 12.35.06.022 Verifying certificate for "" with fingerprint fe:3b:17:xxxxxx and 20 failures
. 2020-03-05 12.35.06.022 Certificate for "" matches cached fingerprint and failures
. 2020-03-05 12.35.06.022 Using TLSv1.2, cipher TLSv1/SSLv3: ECDHE-RSxxxxx, 2048 bit RSA, ECDHExxxxxxx TLSv1.2 Kx=ECDH     Au=RSA  Enc=AESGCM(256) Mac=AEAD
. 2020-03-05 12.35.06.047 TLS connection established. Waiting for welcome message...
< 2020-03-05 12.35.06.047 220 Microsoft FTP Service
> 2020-03-05 12.35.06.047 USER xxxx
< 2020-03-05 12.35.06.047 331 Password required
> 2020-03-05 12.35.06.047 PASS *************
< 2020-03-05 12.35.06.048 530 User cannot log in, home directory inaccessible.
. 2020-03-05 12.35.06.048 Connection failed.
. 2020-03-05 12.35.06.062 Password prompt (last login attempt failed)

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